Analysis of the melon (Cucumis melo) small RNAome by high-throughput pyrosequencing.

ABSTRACT:

Background

Melon (Cucumis melo L.) is a commercially important fruit crop that is cultivated worldwide. The melon research community has recently benefited from the determination of a complete draft genome sequence and the development of associated genomic tools, which have allowed us to focus on small RNAs (sRNAs). These are short, non-coding RNAs 21-24 nucleotides in length with diverse physiological roles. In plants, they regulate gene expression and heterochromatin assembly, and control protection against virus infection. Much remains to be learned about the role of sRNAs in melon.

Results

We constructed 10 sRNA libraries from two stages of developing ovaries, fruits and photosynthetic cotyledons infected with viruses, and carried out high-throughput pyrosequencing. We c

SUBMITTER: Gonzalez-Ibeas D 

PROVIDER: S-EPMC3163571 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature