The type 2C Ser/Thr phosphatase PP2Cgamma is a pre-mRNA splicing factor.

ABSTRACT: To identify activities involved in human pre-mRNA splicing, we have developed a procedure to separate HeLa cell nuclear extract into five complementing fractions. An activity called SCF1 was purified from one of these fractions by assaying for reconstitution of splicing in the presence of the remaining four fractions. A component of SCF1 is shown to be PP2Cgamma, a type 2C Ser/Thr phosphatase of previously unknown function. Previous work suggested that dephosphorylation of splicing factors may be important for catalysis after spliceosome assembly, although the identities of the specific phosphatases involved remain unclear. Here we show that human PP2Cgamma is physically associated with the spliceosome in vitro throughout the splicing reaction, but is first required during the early stages

SUBMITTER: Murray MV 

PROVIDER: S-EPMC316367 | biostudies-literature | 1999 Jan

REPOSITORIES: biostudies-literature