The response of Lactococcus lactis to membrane protein production.

ABSTRACT:

Background

The biogenesis of membrane proteins is more complex than that of water-soluble proteins, and recombinant expression of membrane proteins in functional form and in amounts high enough for structural and functional studies is often problematic. To better engineer cells towards efficient protein production, we set out to understand and compare the cellular consequences of the overproduction of both classes of proteins in Lactococcus lactis, employing a combined proteomics and transcriptomics approach.

Methodology and findings

Highly overproduced and poorly expressed membrane proteins both resulted in severe growth defects, whereas amplified levels of a soluble substrate receptor had no effect. In addition, membrane protein overproduction evoked a general stress respo

SUBMITTER: Marreddy RK 

PROVIDER: S-EPMC3164122 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature