Disrupting the myosin converter-relay interface impairs Drosophila indirect flight muscle performance.

ABSTRACT: Structural interactions between the myosin converter and relay domains have been proposed to be critical for the myosin power stroke and muscle power generation. We tested this hypothesis by mutating converter residue 759, which interacts with relay residues I508, N509, and D511, to glutamate (R759E) and determined the effect on Drosophila indirect flight muscle mechanical performance. Work loop analysis of mutant R759E indirect flight muscle fibers revealed a 58% and 31% reduction in maximum power generation (P(WL)) and the frequency at which maximum power (f(WL)) is generated, respectively, compared to control fibers at 15 °C. Small amplitude sinusoidal analysis revealed a 30%, 36%, and 32% reduction in mutant elastic modulus, viscous modulus, and mechanical rate constant 2πb, respective

SUBMITTER: Ramanath S 

PROVIDER: S-EPMC3164139 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature