Fitting experimental transcription data with a comprehensive template-dependent modular kinetic model.

ABSTRACT: In the companion article, we developed a modular scheme for representing the kinetics of transcription elongation by RNA polymerase. As an example of how to use these approaches, in this article we use a comprehensive modular model of this sort to fit experimental transcript elongation results obtained on the canonical tR2 template of phage λ by means of complementary bulk gel electrophoresis and surface plasmon resonance assays. The gel electrophoresis results, obtained in experiments quenched at various times after initiation of transcription, provide distributions of RNA lengths as a function of time. The surface plasmon resonance methods were used to monitor increases and decreases in the total mass of transcription elongation complexes in the same experiments. The different measures o

SUBMITTER: Greive SJ 

PROVIDER: S-EPMC3164171 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature