A modified FCCS procedure applied to Ly49A-MHC class I cis-interaction studies in cell membranes.

ABSTRACT: The activity of natural killer (NK) cells is regulated by a fine-tuned balance between activating and inhibitory receptors. Dual-color fluorescence cross-correlation spectroscopy (FCCS) was used to directly demonstrate a so-called cis-interaction between a member of the inhibitory NK cell receptor family Ly49 (Ly49A), and its ligand, the major histocompatibility complex (MHC) class I, within the plasma membrane of the same cell. By a refined FCCS model, calibrated by positive and negative control experiments on cells from the same lymphoid cell line, concentrations and diffusion coefficients of free and interacting proteins could be determined on a collection of cells. Using the intrinsic intercellular variation of their expression levels for titration, it was found that the fraction of Ly

SUBMITTER: Stromqvist J 

PROVIDER: S-EPMC3164190 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature