Monoolein lipid phases as incorporation and enrichment materials for membrane protein crystallization.

ABSTRACT: The crystallization of membrane proteins in amphiphile-rich materials such as lipidic cubic phases is an established methodology in many structural biology laboratories. The standard procedure employed with this methodology requires the generation of a highly viscous lipidic material by mixing lipid, for instance monoolein, with a solution of the detergent solubilized membrane protein. This preparation is often carried out with specialized mixing tools that allow handling of the highly viscous materials while minimizing dead volume to save precious membrane protein sample. The processes that occur during the initial mixing of the lipid with the membrane protein are not well understood. Here we show that the formation of the lipidic phases and the incorporation of the membrane protein into

SUBMITTER: Wallace E 

PROVIDER: S-EPMC3164205 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature