Cross-reactivity of ryanodine receptors with plasma membrane ion channel modulators.

ABSTRACT: Various pharmacological agents designed to modulate plasma membrane ion channels seem to significantly affect intracellular Ca²⁺ signaling when acting on their target receptor. Some agents could also cross-react (modulate channels or receptors beyond their putative target) with intracellular Ca²⁺ transporters. This study investigated the potential of thirty putative modulators of either plasma membrane K⁺, Na⁺, or transient receptor potential (TRP) channels to cross-react with intracellular Ca²⁺ release channels [i.e., ryanodine receptors (RyRs)] from skeletal muscle sarcoplasmic reticulum (SR). Screening for cross-reactivity of these various agents was performed by measuring the rate of spontaneous Ca²⁺ leak or caffeine-induced Ca²⁺ release from SR microsomes. Four of the agents displayed

SUBMITTER: Neumann JT 

PROVIDER: S-EPMC3164330 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature