Regulation of exit from mitosis in multinucleate Ashbya gossypii cells relies on a minimal network of genes.

ABSTRACT: In Saccharomyces cerevisiae, mitosis is coupled to cell division by the action of the Cdc fourteen early anaphase release (FEAR) and mitotic exit network (MEN) regulatory networks, which mediate exit from mitosis by activation of the phosphatase Cdc14. The closely related filamentous ascomycete Ashbya gossypii provides a unique cellular setting to study the evolution of these networks. Within its multinucleate hyphae, nuclei are free to divide without the spatial and temporal constraints described for budding yeast. To investigate how this highly conserved system has adapted to these circumstances, we constructed a series of mutants lacking homologues of core components of MEN and FEAR and monitored phenomena such as progression through mitosis and Cdc14 activation. MEN homologues in A. go

SUBMITTER: Finlayson MR 

PROVIDER: S-EPMC3164456 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature