Mi-2/NuRD complex function is required for normal S phase progression and assembly of pericentric heterochromatin.

ABSTRACT: During chromosome duplication, it is essential to replicate not only the DNA sequence, but also the complex nucleoprotein structures of chromatin. Pericentric heterochromatin is critical for silencing repetitive elements and plays an essential structural role during mitosis. However, relatively little is understood about its assembly and maintenance during replication. The Mi2/NuRD chromatin remodeling complex tightly associates with actively replicating pericentric heterochromatin, suggesting a role in its assembly. Here we demonstrate that depletion of the catalytic ATPase subunit CHD4/Mi-2β in cells with a dampened DNA damage response results in a slow-growth phenotype characterized by delayed progression through S phase. Furthermore, we observe defects in pericentric heterochromatin ma

SUBMITTER: Sims JK 

PROVIDER: S-EPMC3164457 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature