Orai1-mediated I (CRAC) is essential for neointima formation after vascular injury.

ABSTRACT:

Rationale

The molecular correlate of the calcium release-activated calcium current (I(CRAC)), the channel protein Orai1, is upregulated in proliferative vascular smooth muscle cells (VSMC). However, the role of Orai1 in vascular disease remains largely unknown.

Objective

The goal of this study was to determine the role of Orai1 in neointima formation after balloon injury of rat carotid arteries and its potential upregulation in a mouse model of VSMC remodeling.

Methods and results

Lentiviral particles encoding short-hairpin RNA (shRNA) targeting either Orai1 (shOrai1) or STIM1 (shSTIM1) caused knockdown of their respective target mRNA and proteins and abrogated store-operated calcium entry and I(CRAC) in VSMC; control shRNA was targeted to luciferase (shLuciferase). B

SUBMITTER: Zhang W 

PROVIDER: S-EPMC3164514 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature