In vitro activity against Staphylococcus aureus of a novel antimicrobial agent, PRF-119, a recombinant chimeric bacteriophage endolysin.

ABSTRACT: Antistaphylococcal activity of the novel chimeric endolysin PRF-119 was evaluated with the microdilution method. The MIC(50) and MIC(90) of 398 methicillin-susceptible Staphylococcus aureus isolates were 0.098 ?g/ml and 0.391 ?g/ml, respectively (range, 0.024 to 0.780 ?g/ml). Both the MIC(50) and MIC(90) values of 776 methicillin-resistant S. aureus isolates were 0.391 ?g/ml (range, 0.024 to 1.563 ?g/ml). All 192 clinical isolates of coagulase-negative staphylococci exhibited MIC values of >50 ?g/ml. In conclusion, PRF-119 exhibited very good activity specifically against S. aureus.

SUBMITTER: Idelevich EA 

PROVIDER: S-EPMC3165309 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature