Poly-3-hydroxybutyrate metabolism in the type II methanotroph Methylocystis parvus OBBP.

ABSTRACT: Differences in carbon assimilation pathways and reducing power requirements among organisms are likely to affect the role of the storage polymer poly-3-hydroxybutyrate (PHB). Previous researchers have demonstrated that PHB functions as a sole growth substrate in aerobic cultures enriched on acetate during periods of carbon deficiency, but it is uncertain how C(1) metabolism affects the role of PHB. In the present study, the type II methanotroph Methylocystis parvus OBBP did not replicate using stored PHB in the absence of methane, even when all other nutrients were provided in excess. When PHB-rich cultures of M. parvus OBBP were deprived of carbon and nitrogen for 48 h, they did not utilize significant amounts of stored PHB, and neither cell concentrations nor concentrations of total susp

SUBMITTER: Pieja AJ 

PROVIDER: S-EPMC3165381 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature