Transcription analysis of a lantibiotic gene cluster from Bifidobacterium longum DJO10A.

ABSTRACT: Bifidobacterium longum DJO10A was previously demonstrated to produce a lantibiotic, but only during growth on agar media. To evaluate the feasibility of production of this lantibiotic in broth media, a transcription analysis of the lanA gene was undertaken. Comparative microarray analysis of broth and agar cultures of B. longum DJO10A revealed that the lantibiotic production, modification, transport/peptidase, and immunity genes were significantly upregulated in agar cultures, while the two-component regulatory genes were expressed equally under both conditions. This suggested that the signal transduction regulatory system should function in broth cultures. Real-time PCR and Northern hybridization confirmed that lanA gene expression was significantly repressed in broth cultures. A crude la

SUBMITTER: Lee JH 

PROVIDER: S-EPMC3165390 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature