Potential of pmoA amplicon pyrosequencing for methanotroph diversity studies.

ABSTRACT: We analyzed the potential of pmoA amplicon pyrosequencing compared to that of Sanger sequencing with paddy soils as a model environment. We defined operational taxonomic unit (OTU) cutoff values of 7% and 18%, reflecting methanotrophic species and major phylogenetic pmoA lineages, respectively. Major lineages were already well covered by clone libraries; nevertheless, pyrosequencing provided a higher level of diversity at the species level.

SUBMITTER: Luke C 

PROVIDER: S-EPMC3165393 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature