Geographic distribution of secondary metabolite genes in the marine actinomycete Salinispora arenicola.

ABSTRACT: The molecular fingerprinting technique terminal-restriction fragment length polymorphism (T-RFLP) was used in combination with sequence-based approaches to evaluate the geographic distribution of secondary metabolite biosynthetic genes in strains of the marine actinomycete Salinispora arenicola. This study targeted ketosynthase (KS) domains from type I polyketide synthase (PKS) genes and revealed four distinct clusters, the largest of which was comprised of strains from all six global locations sampled. The remaining strains fell into three smaller clusters comprised of strains derived entirely from the Red Sea, the Sea of Cortez, or around the Island of Guam. These results reveal variation in the secondary metabolite gene collectives maintained by strains that are largely clonal at the 16

SUBMITTER: Edlund A 

PROVIDER: S-EPMC3165397 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature