Novel multiplex single nucleotide polymorphism-based method for identifying epidemic clones of Listeria monocytogenes.

ABSTRACT: A novel primer extension-based, multiplex minisequencing assay targeting six highly informative single nucleotide polymorphisms (SNPs) in four virulence genes correctly identified and differentiated all four epidemic clones (ECs) of Listeria monocytogenes and 9 other strains initially misclassified as non-ECs. This assay allows rapid, accurate, and high-throughput screening for all known ECs of L. monocytogenes.

SUBMITTER: Lomonaco S 

PROVIDER: S-EPMC3165415 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature