Effects of fluconazole on the secretome, the wall proteome, and wall integrity of the clinical fungus Candida albicans.

ABSTRACT: Fluconazole is a commonly used antifungal drug that inhibits Erg11, a protein responsible for 14α-demethylation during ergosterol synthesis. Consequently, ergosterol is depleted from cellular membranes and replaced by toxic 14α-methylated sterols, which causes increased membrane fluidity and drug permeability. Surface-grown and planktonic cultures of Candida albicans responded similarly to fluconazole at 0.5 mg/liter, showing reduced biomass formation, severely reduced ergosterol levels, and almost complete inhibition of hyphal growth. There was no evidence of cell leakage. Mass spectrometric analysis of the secretome showed that its composition was strongly affected and included 17 fluconazole-specific secretory proteins. Relative quantification of (14)N-labeled query walls relative to a

SUBMITTER: Sorgo AG 

PROVIDER: S-EPMC3165447 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature