Evaluation of high-throughput sequencing for identifying known and unknown viruses in biological samples.

ABSTRACT: High-throughput sequencing furnishes a large number of short sequence reads from uncloned DNA and has rapidly become a major tool for identifying viruses in biological samples, and in particular when the target sequence is undefined. In this study, we assessed the analytical sensitivity of a pipeline for detection of viruses in biological samples based on either the Roche-454 genome sequencer or Illumina genome analyzer platforms. We sequenced biological samples artificially spiked with a wide range of viruses with genomes composed of single or double-stranded DNA or RNA, including linear or circular single-stranded DNA. Viruses were added at a very low concentration most often corresponding to 3 or 0.8 times the validated level of detection of quantitative reverse transcriptase PCRs (RT-P

SUBMITTER: Cheval J 

PROVIDER: S-EPMC3165575 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature