Overexpression of cloned RhsA sequences perturbs the cellular translational machinery in Escherichia coli.

ABSTRACT: RhsA is a member of the multigene Rhs family and consists of a complex genetic sequence. This sequence consists of several distinct components, including a GC-rich core (core open reading frame [ORF]), an AT-rich extension (ext-a1) of the core ORF and an AT-rich region following the core extension (dsORF-a1). The functions of RhsA and the different distinct components, which can include open reading frames, are not well understood. Here, we study the effect of overexpression of the ext-a1 sequence and the ext-a1 3' region, which includes a partial sequence of dsORF-a1, on Escherichia coli cells. Cells expressing these sequences show reduced cell growth and cell viability. The expression of these sequences dramatically affects different components of the transcription and translation machin

SUBMITTER: Aggarwal K 

PROVIDER: S-EPMC3165670 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature