Establishment and characterization of Prnp knockdown neuroblastoma cells using dual microRNA-mediated RNA interference.

ABSTRACT: Prion diseases are fatal transmissible neurodegenerative disorders. In the pathogenesis of the disease, the cellular prion protein (PrPC) is required for replication of abnormal prion (PrPSc), which results in accumulation of PrPSc. Although there have been extensive studies using Prnp knockout systems, the normal function of PrPC remains ambiguous. Compared with conventional germline knockout technologies and transient naked siRNA-dependent knockdown systems, newly constructed durable chained-miRNA could provide a cell culture model that is closer to the disease status and easier to achieve with no detrimental sequelae. The selective silencing of a target gene by RNA interference (RNAi) is a powerful approach to investigate the unknown function of genes in vitro and in vivo. To reduce PrP

SUBMITTER: Kang SG 

PROVIDER: S-EPMC3166508 | biostudies-literature | 2011 Apr-Jun

REPOSITORIES: biostudies-literature