Transcriptome profiling of sheep granulosa cells and oocytes during early follicular development obtained by laser capture microdissection.

ABSTRACT:

Background

Successful achievement of early folliculogenesis is crucial for female reproductive function. The process is finely regulated by cell-cell interactions and by the coordinated expression of genes in both the oocyte and in granulosa cells. Despite many studies, little is known about the cell-specific gene expression driving early folliculogenesis. The very small size of these follicles and the mixture of types of follicles within the developing ovary make the experimental study of isolated follicular components very difficult.The recently developed laser capture microdissection (LCM) technique coupled with microarray experiments is a promising way to address the molecular profile of pure cell populations. However, one main challenge was to preserve the RNA quality during t

SUBMITTER: Bonnet A 

PROVIDER: S-EPMC3166951 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature