Mass spectrometric-based proteomic analysis of amyloid neuropathy type in nerve tissue.

ABSTRACT:

Objective

To determine the specific type of amyloid from nerve biopsies using laser microdissection (LMD) and mass spectrometric (MS)-based proteomic analysis.

Design, setting, and patients

Twenty-one nerve biopsy specimens (17 sural, 3 sciatic, and 1 root amyloidoma) infiltrated by amyloid were studied. Immunohistochemical subtyping was unable to determine the specific amyloid type for these 21 cases, but the clinical diagnosis was made based on additional testing. Clinical diagnosis was made through evaluation of serum monoclonal proteins, biopsy of bone marrow for acquired monoclonal immunoglobulin light chain amyloidosis, and kindred evaluations with DNA sequencing of transthyretin (TTR) and gelsolin (GSN) genes. Our study included 8 cases of acquired monoclonal immunogl

SUBMITTER: Klein CJ 

PROVIDER: S-EPMC3167172 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature