Two-dimensional difference gel electrophoresis (DIGE) analysis of sera from visceral leishmaniasis patients.

ABSTRACT:

Introduction

Visceral leishmaniasis is a parasitic infection caused by Lesihmania donovani complex and transmitted by the bite of the phlebotomine sand fly. It is an endemic disease in many developing countries with more than 90% of the cases occurring in Bangladesh, India, Nepal, Sudan, Ethiopia and Brazil. The disease is fatal if untreated. The disease is conventionally diagnosed by demonstrating the intracellular parasite in bone marrow or splenic aspirates. This study was carried out to discover differentially expressed proteins which could be potential biomarkers.

Methods

Sera from six visceral leishmaniasis patients and six healthy controls were depleted of high abundant proteins by immunodepletion. The depleted sera were compared by 2-D Difference in gel electrophores

SUBMITTER: Rukmangadachar LA 

PROVIDER: S-EPMC3167202 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature