Transcripts that associate with the RNA binding protein, DEAD-END (DND1), in embryonic stem (ES) cells.

ABSTRACT:

Background

The RNA binding protein, DEAD END (DND1), is essential for maintaining viable germ cells in vertebrates. It is also a testicular germ cell tumor susceptibility factor in mice. DND1 has been shown to interact with the 3'-untranslated region (3'-UTR) of mRNAs such as P27 and LATS2. Binding of DND1 to the 3'-UTRs of these transcripts blocks the inhibitory function of microRNAs (miRNA) from these transcripts and in this way DND1 helps maintain P27 and LATS2 protein expression. We found that DND1 is also expressed in embryonic stem (ES) cells. Because ES cells share similar gene expression patterns as germ cells, we utilized ES cells to identify additional candidate mRNAs that associate with DND1.

Results

ES cells are readily amenable to genetic modification and easier

SUBMITTER: Zhu R 

PROVIDER: S-EPMC3167746 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature