DNA binding compatibility of the Streptococcus pneumoniae SsbA and SsbB proteins.

ABSTRACT:

Background

Streptococcus pneumoniae has two paralogous, homotetrameric, single-stranded DNA binding (SSB) proteins, designated SsbA and SsbB. Previous studies demonstrated that SsbA and SsbB have different solution-dependent binding mode preferences with variable DNA binding capacities. The impact of these different binding properties on the assembly of multiple SsbAs and SsbBs onto single-stranded DNA was investigated.

Methodology/principal findings

The complexes that were formed by the SsbA and SsbB proteins on dT(n) oligomers of defined lengths were examined by polyacrylamide gel electrophoresis. Complexes containing either two SsbAs or two SsbBs, or mixed complexes containing one SsbA and one SsbB, could be formed readily, provided the dT(n) oligomer was long enough to s

SUBMITTER: Salerno B 

PROVIDER: S-EPMC3168475 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature