A systematic analysis of eluted fraction of plasma post immunoaffinity depletion: implications in biomarker discovery.

ABSTRACT: Plasma is the most easily accessible source for biomarker discovery in clinical proteomics. However, identifying potential biomarkers from plasma is a challenge given the large dynamic range of proteins. The potential biomarkers in plasma are generally present at very low abundance levels and hence identification of these low abundance proteins necessitates the depletion of highly abundant proteins. Sample pre-fractionation using immuno-depletion of high abundance proteins using multi-affinity removal system (MARS) has been a popular method to deplete multiple high abundance proteins. However, depletion of these abundant proteins can result in concomitant removal of low abundant proteins. Although there are some reports suggesting the removal of non-targeted proteins, the predominant view

SUBMITTER: Yadav AK 

PROVIDER: S-EPMC3168506 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature