Localization-dependent translation requires a functional interaction between the 5' and 3' ends of oskar mRNA.

ABSTRACT: The precise restriction of proteins to specific domains within a cell plays an important role in early development and differentiation. An efficient way to localize and concentrate proteins is by localization of mRNA in a translationally repressed state, followed by activation of translation when the mRNA reaches its destination. A central issue is how localized mRNAs are derepressed. In this study we demonstrate that, when oskar mRNA reaches the posterior pole of the Drosophila oocyte, its translation is derepressed by an active process that requires a specific element in the 5' region of the mRNA. We demonstrate that this novel type of element is a translational derepressor element, whose functional interaction with the previously identified repressor region in the oskar 3' UTR is requir

SUBMITTER: Gunkel N 

PROVIDER: S-EPMC316867 | biostudies-literature | 1998 Jun

REPOSITORIES: biostudies-literature