Folate-targeted nanoparticles show efficacy in the treatment of inflammatory arthritis.

ABSTRACT:

Objective

To investigate the uptake of a poly(amidoamine) dendrimer (generation 5 [G5]) nanoparticle covalently conjugated to polyvalent folic acid (FA) as the targeting ligand into macrophages, and to investigate the activity of an FA- and methotrexate (MTX)-conjugated dendrimer (G5-FA-MTX) as a therapeutic for the inflammatory disease of arthritis.

Methods

In vitro studies were performed in macrophage cell lines and in isolated mouse macrophages to check the cellular uptake of fluorescence-tagged G5-FA nanoparticles, using flow cytometry and confocal microscopy. In vivo studies were conducted in a rat model of collagen-induced arthritis to evaluate the therapeutic potential of G5-FA-MTX.

Results

Folate-targeted dendrimer bound and internalized in a receptor-specific

SUBMITTER: Thomas TP 

PROVIDER: S-EPMC3168725 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature