A cost-effective ELP-intein coupling system for recombinant protein purification from plant production platform.

ABSTRACT:

Background

Plant bioreactor offers an efficient and economical system for large-scale production of recombinant proteins. However, high cost and difficulty in scaling-up of downstream purification of the target protein, particularly the common involvement of affinity chromatography and protease in the purification process, has hampered its industrial scale application, therefore a cost-effective and easily scale-up purification method is highly desirable for further development of plant bioreactor.

Methodology/principal findings

To tackle this problem, we investigated the ELP-intein coupling system for purification of recombinant proteins expressed in transgenic plants using a plant lectin (PAL) with anti-tumor bioactivity as example target protein and rice seeds as producti

SUBMITTER: Tian L 

PROVIDER: S-EPMC3168869 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature