A regression system for estimation of errors introduced by confocal imaging into gene expression data in situ.

ABSTRACT:

Background

Accuracy of the data extracted from two-dimensional confocal images is limited due to experimental errors that arise in course of confocal scanning. The common way to reduce the noise in images is sequential scanning of the same specimen several times with the subsequent averaging of multiple frames. Attempts to increase the dynamical range of an image by setting too high values of microscope PMT parameters may cause clipping of single frames and introduce errors into the data extracted from the averaged images. For the estimation and correction of this kind of errors a method based on censoring technique (Myasnikova et al., 2009) is used. However, the method requires the availability of all the confocal scans along with the averaged image, which is normally not provided

SUBMITTER: Myasnikova E 

PROVIDER: S-EPMC3169536 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature