Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR.

ABSTRACT: Fungi constitute an important group in soil biological diversity and functioning. However, characterization and knowledge of fungal communities is hampered because few primer sets are available to quantify fungal abundance by real-time quantitative PCR (real-time Q-PCR). The aim in this study was to quantify fungal abundance in soils by incorporating, into a real-time Q-PCR using the SYBRGreen® method, a primer set already used to study the genetic structure of soil fungal communities. To satisfy the real-time Q-PCR requirements to enhance the accuracy and reproducibility of the detection technique, this study focused on the 18S rRNA gene conserved regions. These regions are little affected by length polymorphism and may provide sufficiently small targets, a crucial criterion for enhancing

SUBMITTER: Chemidlin Prevost-Boure N 

PROVIDER: S-EPMC3169588 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature