Protein tyrosine phosphatase non-receptor type 14 is a novel sperm-motility biomarker.

ABSTRACT:

Purpose

To understand the molecular basis of sperm-motility and to identify related novel motility biomarkers.

Methods

Two-dimensional electrophoresis (2DE) followed by Reverse-phase-nano-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (RP-nano-HPLC-ESI-MS/MS) were applied to establish the human sperm proteome. Then the sperm proteome of moderate-motile human sperm fraction and that of good-motile human sperm fraction from pooled spermatozoa of forty normozoospermic donors (Group 1 subjects) were compared to identify the dysregulated proteins. Among these down-regulated proteins, Protein tyrosine phosphatase non-receptor type 14 (PTPN14) was chosen to reconfirm by Western blotting and semi-quantitative reverse transcription polymerase

SUBMITTER: Chao HC 

PROVIDER: S-EPMC3169687 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature