The relationship between transcription initiation RNAs and CCCTC-binding factor (CTCF) localization.

ABSTRACT:

Background

Transcription initiation RNAs (tiRNAs) are nuclear localized 18 nucleotide RNAs derived from sequences immediately downstream of RNA polymerase II (RNAPII) transcription start sites. Previous reports have shown that tiRNAs are intimately correlated with gene expression, RNA polymerase II binding and behaviors, and epigenetic marks associated with transcription initiation, but not elongation.

Results

In the present work, we show that tiRNAs are commonly found at genomic CCCTC-binding factor (CTCF) binding sites in human and mouse, and that CTCF sites that colocalize with RNAPII are highly enriched for tiRNAs. To directly investigate the relationship between tiRNAs and CTCF we examined tiRNAs originating near the intronic CTCF binding site in the human tumor suppres

SUBMITTER: Taft RJ 

PROVIDER: S-EPMC3170176 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature