Comparative analyses reveal discrepancies among results of commonly used methods for Anopheles gambiaemolecular form identification.

ABSTRACT:

Background

Anopheles gambiae M and S molecular forms, the major malaria vectors in the Afro-tropical region, are ongoing a process of ecological diversification and adaptive lineage splitting, which is affecting malaria transmission and vector control strategies in West Africa. These two incipient species are defined on the basis of single nucleotide differences in the IGS and ITS regions of multicopy rDNA located on the X-chromosome. A number of PCR and PCR-RFLP approaches based on form-specific SNPs in the IGS region are used for M and S identification. Moreover, a PCR-method to detect the M-specific insertion of a short interspersed transposable element (SINE200) has recently been introduced as an alternative identification approach. However, a large-scale comparative analysis o

SUBMITTER: Santolamazza F 

PROVIDER: S-EPMC3170251 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature