Gene expression profiling reveals new potential players of gonad differentiation in the chicken embryo.

ABSTRACT:

Background

In birds as in mammals, a genetic switch determines whether the undifferentiated gonad develops into an ovary or a testis. However, understanding of the molecular pathway(s) involved in gonad differentiation is still incomplete.

Methodology/principal findings

With the aim of improving characterization of the molecular pathway(s) involved in gonad differentiation in the chicken embryo, we developed a large scale real time reverse transcription polymerase chain reaction approach on 110 selected genes for evaluation of their expression profiles during chicken gonad differentiation between days 5.5 and 19 of incubation. Hierarchical clustering analysis of the resulting datasets discriminated gene clusters expressed preferentially in the ovary or the testis, and/or at

SUBMITTER: Carre GA 

PROVIDER: S-EPMC3170287 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature