Selection in coastal Synechococcus (cyanobacteria) populations evaluated from environmental metagenomes.

ABSTRACT: Environmental metagenomics provides snippets of genomic sequences from all organisms in an environmental sample and are an unprecedented resource of information for investigating microbial population genetics. Current analytical methods, however, are poorly equipped to handle metagenomic data, particularly of short, unlinked sequences. A custom analytical pipeline was developed to calculate dN/dS ratios, a common metric to evaluate the role of selection in the evolution of a gene, from environmental metagenomes sequenced using 454 technology of flow-sorted populations of marine Synechococcus, the dominant cyanobacteria in coastal environments. The large majority of genes (98%) have evolved under purifying selection (dN/dS<1). The metagenome sequence coverage of the reference genomes was no

SUBMITTER: Tai V 

PROVIDER: S-EPMC3170327 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature