Automated biochemical, morphological, and organizational assessment of precancerous changes from endogenous two-photon fluorescence images.

ABSTRACT:

Background

Multi-photon fluorescence microscopy techniques allow for non-invasive interrogation of live samples in their native environment. These methods are particularly appealing for identifying pre-cancers because they are sensitive to the early changes that occur on the microscopic scale and can provide additional information not available using conventional screening techniques.

Methodology/principal findings

In this study, we developed novel automated approaches, which can be employed for the real-time analysis of two-photon fluorescence images, to non-invasively discriminate between normal and pre-cancerous/HPV-immortalized engineered tissues by concurrently assessing metabolic activity, morphology, organization, and keratin localization. Specifically, we found that

SUBMITTER: Levitt JM 

PROVIDER: S-EPMC3170385 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature