Engineering of an E. coli outer membrane protein FhuA with increased channel diameter.

ABSTRACT:

Background

Channel proteins like FhuA can be an alternative to artificial chemically synthesized nanopores. To reach such goals, channel proteins must be flexible enough to be modified in their geometry, i.e. length and diameter. As continuation of a previous study in which we addressed the lengthening of the channel, here we report the increasing of the channel diameter by genetic engineering.

Results

The FhuA Δ1-159 diameter increase has been obtained by doubling the amino acid sequence of the first two N-terminal β-strands, resulting in variant FhuA Δ1-159 Exp. The total number of β-strands increased from 22 to 24 and the channel surface area is expected to increase by ~16%. The secondary structure analysis by circular dichroism (CD) spectroscopy shows a high β-sheet cont

SUBMITTER: Krewinkel M 

PROVIDER: S-EPMC3170585 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature