Repression of KIAA1199 attenuates Wnt-signalling and decreases the proliferation of colon cancer cells.

ABSTRACT:

Background

The KIAA1199 transcript is upregulated in colon adenomas and downregulated upon β-catenin knockdown.

Methods

Transcript profiling was performed on >500 colon biopsies, methylation profiling data were compared with transcript data. Immunohistochemistry assessed KIAA1199 protein expression in 270 stage II/III tumours (>3 years follow-up). The effects of stable KIAA1199 knockdown in SW480 cells (three different constructs) were studied using transcriptional profiling, proliferation and protein analysis.

Results

The KIAA1199 transcript was strongly upregulated in 95% of adenocarcinomas. Absent expression in normal mucosa correlated with KIAA1199 promotor methylation. Nuclear and cytoplasmic KIAA1199 protein expression was identified in colon adenocarcinomas and

SUBMITTER: Birkenkamp-Demtroder K 

PROVIDER: S-EPMC3170968 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature