An acetylcholine receptor lacking both γ and ε subunits mediates transmission in zebrafish slow muscle synapses.

ABSTRACT: Fast and slow skeletal muscle types in larval zebrafish can be distinguished by a fivefold difference in the time course of their synaptic decay. Single-channel recordings indicate that this difference is conferred through kinetically distinct nicotinic acetylcholine receptor (AChR) isoforms. The underlying basis for this distinction was explored by cloning zebrafish muscle AChR subunit cDNAs and expressing them in Xenopus laevis oocytes. Measurements of single-channel conductance and mean open burst duration assigned α(2)βδε to fast muscle synaptic current. Contrary to expectations, receptors composed of only αβδ subunits (presumed to be α(2)βδ(2) receptors) recapitulated the kinetics and conductance of slow muscle single-channel currents. Additional evidence in support of γ/ε-less recept

SUBMITTER: Mongeon R 

PROVIDER: S-EPMC3171075 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature