High-speed shaking of frozen blood clots for extraction of human and malaria parasite DNA.

ABSTRACT:

Background

Frozen blood clots remaining after serum collection is an often disregarded source of host and pathogen DNA due to troublesome handling and suboptimal outcome.

Methods

High-speed shaking of clot samples in a cell disruptor manufactured for homogenization of tissue and faecal specimens was evaluated for processing frozen blood clots for DNA extraction. The method was compared to two commercial clot protocols based on a chemical kit and centrifugation through a plastic sieve, followed by the same DNA extraction protocol. Blood clots with different levels of parasitaemia (1-1,000 p/μl) were prepared from parasite cultures to assess sensitivity of PCR detection. In addition, clots retrieved from serum samples collected within two epidemiological studies in Kenya (n =

SUBMITTER: Lundblom K 

PROVIDER: S-EPMC3171377 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature