Comparison of genome-wide gene expression in suture- and alkali burn-induced murine corneal neovascularization.

ABSTRACT:

Purpose

Suture placement and alkali burn to the cornea are often used to induce inflammatory corneal neovascularization (CorNV) models in animals. This study compares the changes in genome-wide gene expression under these two CorNV conditions in mice.

Methods

CorNV were induced in Balb/c mice by three interrupted 10-0 sutures placed at sites about 1 mm from the corneal apex, or by alkali burns that were 2 mm in size in the central area of the cornea. At the points in time when neovascularization progressed most quickly, some eyeballs were subjected to histological staining to examine CorNV and inflammatory cells infiltration, and some corneas were harvested to extract mRNA for microarray assay. After normalization and filtering, the microarray data were subject to statistica

SUBMITTER: Jia C 

PROVIDER: S-EPMC3171500 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature