Inhibition of the signalling kinase JAK3 alleviates inflammation in monoarthritic rats.

ABSTRACT:

Background and purpose

Many cytokines associated with autoimmune disorders and inflammation have been shown to activate the signalling kinase JAK3, implying that JAK3 plays key roles in the pathogenesis of these diseases. Therefore, investigating the alterations of JAK3 activity and the efficacy of selective JAK3 antagonists in animal models of such disorders is essential to a better understanding of the biology of JAK3 and to assess the potential clinical benefits of JAK3 inhibitors.

Experimental approach

Through high-throughput cell-based screening using the NCI compound library, we identified NSC163088 (berberine chloride) as a novel inhibitor of JAK3. Specificity and efficacy of this compound were investigated in both cellular and animal models.

Key results

We sho

SUBMITTER: Kim BH 

PROVIDER: S-EPMC3171864 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature