Optimal cutoff point for immunoperoxidase detection of C4d in the renal allograft: results from a multicenter study.

ABSTRACT:

Background

Although C4d deposition in peritubular capillaries has been identified as a strong risk factor for subsequent renal allograft loss, the optimal cutoff for the fraction of peritubular capillaries needed to establish a positive stain in formalin-fixed, paraffin-embedded material has not been defined systematically. The objective of this study was to establish the threshold for positive staining that best predicts renal outcome in renal biopsies in a multicenter study in which local and central pathologic conditions were compared.

Methods

Unstained renal biopsy slides were obtained from 296 patients. The percentage of peritubular capillaries staining positively for C4d was detected by immunoperoxidase staining.

Results

The percentage C4d deposition ranged from

SUBMITTER: Crary GS 

PROVIDER: S-EPMC3171966 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature