Antibiotic and antiseptic resistance genes are linked on a novel mobile genetic element: Tn6087.

ABSTRACT:

Objectives

Tn916-like elements are one of the most common types of integrative and conjugative element (ICE). In this study we aimed to determine whether novel accessory genes, i.e. genes whose products are not involved in mobility or regulation, were present on a Tn916-like element (Tn6087) isolated from Streptococcus oralis from the human oral cavity.

Methods

A minocycline-resistant isolate was analysed using restriction fragment length polymorphism (RFLP) analysis on amplicons derived from Tn916 and DNA sequencing to determine whether there were genetic differences in Tn6087 compared with Tn916. Mutational analysis was used to determine whether the novel accessory gene found was responsible for an observed extra phenotype.

Results

A novel Tn916-like element, Tn6087

SUBMITTER: Ciric L 

PROVIDER: S-EPMC3172042 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature