Isolation of functional mitochondria from rat kidney and skeletal muscle without manual homogenization.

ABSTRACT: Isolation of functional and intact mitochondria from solid tissue is crucial for studies that focus on the elucidation of normal mitochondrial physiology and/or mitochondrial dysfunction in conditions such as aging, diabetes, and cancer. There is growing recognition of the importance of mitochondria both as targets for drug development and as off-target mediators of drug side effects. Unfortunately, mitochondrial isolation from tissue is generally carried out using homogenizer-based methods that require extensive operator experience to obtain reproducible high-quality preparations. These methods limit dissemination, impede scale-up, and contribute to difficulties in reproducing experimental results over time and across laboratories. Here we describe semiautomated methods to disrupt tissue

SUBMITTER: Gross VS 

PROVIDER: S-EPMC3172370 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature