Replication of a universal nucleobase provides unique insight into the role of entropy during DNA polymerization and pyrophosphorolysis.

ABSTRACT: Most models accounting for the efficiency and fidelity of DNA polymerization invoke the use of either hydrogen bonding contacts or complementarity of shape and size between the formed base pair. This report evaluates these mechanisms by quantifying the ability of a high-fidelity DNA polymerase to replicate 5-nitroindole, a purine mimetic devoid of classic hydrogen bonding capabilities. 5-NITP acts as a universal nucleotide since it is incorporated opposite any of the four natural nucleobases with nearly equal efficiencies. Surprising, the polymerization reaction is not reciprocal as natural nucleotides are poorly incorporated opposite 5-nitroindole in the template strand. Incorporation opposite 5-nitroindole is more efficient using natural nucleotides containing various modifications that

SUBMITTER: Zhang X 

PROVIDER: S-EPMC3172712 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature