DNA methylation in the arginase-nitric oxide synthase pathway is associated with exhaled nitric oxide in children with asthma.

ABSTRACT:

Rationale

Genetic variation in arginase (ARG) and nitric oxide synthase (NOS) has been associated with exhaled nitric oxide (FeNO) levels in children. Little is known about whether epigenetic variation in these genes modulates FeNO.

Objectives

To evaluate whether DNA methylation in ARG and NOS genes is associated with FeNO.

Methods

A subset of 940 participants in the Children's Health Study were selected for this study. Children were eligible if they had FeNO measurements and buccal cells collected on the same day. CpG loci located in the promoter regions of NOS1, NOS2A, NOS3, ARG1, and ARG2 genes were analyzed. Multiple loci in each gene were evaluated individually and averaged together. DNA methylation was measured using a bisulfite-polymerase chain reaction pyroseq

SUBMITTER: Breton CV 

PROVIDER: S-EPMC3172885 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature