Cyclooxygenase-2 regulates Th17 cell differentiation during allergic lung inflammation.

ABSTRACT:

Rationale

Th17 cells comprise a distinct lineage of proinflammatory T helper cells that are major contributors to allergic responses. It is unknown whether cyclooxygenase (COX)-derived eicosanoids regulate Th17 cells during allergic lung inflammation.

Objectives

To determine the role of COX metabolites in regulating Th17 cell differentiation and function during allergic lung inflammation.

Methods

COX-1(-/-), COX-2(-/-), and wild-type mice were studied in an in vivo model of ovalbumin-induced allergic inflammation and an in vitro model of Th17 differentiation using flow cytometry, cytokine assays, confocal microscopy, real-time polymerase chain reaction, and immunoblotting. In addition, the role of specific eicosanoids and their receptors was examined using synthetic p

SUBMITTER: Li H 

PROVIDER: S-EPMC3172888 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature